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Genetic monitoring of Eurasian beaver (Castor fiber) in Switzerland and implications for the management of the species
Minnig S., Angst C., Jacob G.
P. 20-27
Eurasian beaver (Castor fiber) has been reintroduced in Switzerland between 1956 and 1977. Individuals from the refugium population in France (C. f. galliae) were released in the Rhone catchment area and in tributaries of the Rhine catchment area in Western Switzerland. Individuals from the refugium populations from Norway (C. f. fiber) and Russia (C. f. orientoeuropaeus) were released in tributaries of the Rhine catchment in Eastern Switzerland. In the Rhine basin beavers of different origins came into contact. This study provides a first evaluation of the reintroduction program of beaver in Switzerland and gives implications for the post-release genetic management of the Swiss beaver population. We report on the genetic monitoring of the beaver population in Switzerland, based on the analysis of 251 dead found individuals collected from 1998 to 2014 and a combination of mitochondrial and nuclear genetic markers. We found no evidence of the presence of North American beaver (Castor canadensis) and we observed three mitochondrial DNA haplotypes, assigned to the refugium populations in France (C. f. galliae), Norway (C. f. fiber) and Germany (C. f. albicus). Based on the analysis of seven microsatellite loci, we inferred that the beaver population in Switzerland consists of two genetic clusters and we found evidence of a zone of secondary contact. We observed low levels of genetic diversity and we could show that individuals separated by distances up to 50 km were closely related.


  • Angst C. 2010. Mit dem Biber leben. Bestandeserhebung 2008. Perspektiven für den Umgang mit dem Biber in der Schweiz. Bundesamt für Umwelt, Bern, und Schweizer Zentrum für die Kartographie der Fauna, Neuenburg, Umwelt-Wissen, No.1008. 156 S.
  • BAFU 2016. Konzept Biber Schweiz. Vollzugshilfe des BAFU zum Bibermanagement in der Schweiz. Federal Office for the Environment. 43 S.
  • Bergl R.A. & Vigilant L. 2006. Genetic analysis reveals population structure and recent migration within the highly fragmented range of the Cross River gorilla (Gorilla gorilla diehli) // Molecular Ecology. Vol.16. No.3. P.501–516.
  • Biedrzycka A., Konior B., Babik W., Swislocka M. & Ratkiewicz M. 2014. Admixture of two phylogeographic lineages of the Eurasian beaver in Poland // Mammalian Biology. Vol.79. P.287–296.
  • Crawford J., Liu Z., Nelson T., Nielsen C. & Bloomquist C. 2008. Isolation and characterization of microsatellite loci in the beaver Castor canadensis // Molecular Ecology Resources. Vol.8. P.616–618.
  • Dewas M., Herr J., Schley L., Angst C., Manet B., Landry P. & Catusse M. 2012. Recovery and status of native and introduced beavers Castor fiber and Castor canadensis in France and neighboring countries // Mammal Review. Vol.42. P.144–165.
  • Ducroz J.F., Stubbe M., Saveljev A.P., Heidecke D., Samjaa R., Uleviиius A., Stubbe A. & Durka W. 2005. Genetic variation and population structure of the Eurasian beaver Castor fiber in Eastern Europe and Asia // Journal of Mammalogy. Vol.86. No.6. P.1059–1067.
  • Duelli P. 1994. Rote Listen der gefährdeten Tierarten der Schweiz. Bern: Bundesamt für Umwelt, Wald und Landschaft. 93 S.
  • Durka W., Babik W., Ducroz J.F., Hiedecke D., Rosell F., Samjaa R., Saveljev A.P., Stubbe A., Uleviиius A. & Stubbe M. 2005. Mitochondrial phylogeography of the Eurasian beaver Castor fiber L. // Molecular Ecology. Vol.14. No.12. P.3843–3856.
  • ESRI 2011. ArcGIS Desktop: Release 10. Redlands, CA: Environmental Systems Research Institute.
  • Frosch C., Kraus R.H.S., Angst C., Allgцwer R., Michaux J., Teubner J. & Nowak C. 2014. The genetic legacy of multiple beaver reintroductions in Central Europe // PLoS ONE. No.9(5): e97619.
  • Goossens B., Waits L.P. & Taberlet P. 1998. Plucked hair samples as a source of DNA: reliability of dinucleotide microsatellite genotyping // Molecular Ecology. Vol.7. P.1237–1241.
  • Grossenbacher K. 2005. 40 Jahre Biber Castor fiber im Kanton Bern und angrenzenden Gebieten Beobachtungen 1996–2005 // Mitteilungen der Naturforschenden Gesellschaft. Bd.62. S.54–106.
  • Hall T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows // Nucleic Acids Symposium Series. No.41. P.95–98.
  • Halley D.J. 2011. Sourcing Eurasian beaver Castor fiber stock for reintroductions in Great Britain and Western Europe // Mammal Review. Vol.41. P.40–53.
  • Halley D., Rosell F. & Saveljev A. 2012. Population and distribution of Eurasian beaver Castor fiber // Baltic Forestry. Vol.18. No.1. P.168–175.
  • IUCN/SSC. 2013. Guidelines for Reintroductions and Other Conservation Translocations. Version 1.0. Gland, Switzerland: IUCN Species Survival Commission. 57 p.
  • Moritz C. 1994. Applications of mitochondrial DNA analysis in conservation – a critical review // Molecular Ecology. Vol.3. P.401–411.
  • Nei M. & Kumar S. 2000. Molecular Evolution and Phylogenetics. Oxford: Oxford University Press. 352 p.
  • Peakall R. & Smouse P.E. 2006. GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research // Molecular Ecology Notes. Vol.6. P.288–295.
  • Pelz-Serrano K., Munguia-Vega A., Piaggio A.J., Neubaum M., Munclinger P., Partl A., Van Piper C. & Culver M. 2009. Development of nine new microsatellite loci for the American beaver, Castor canadensis (Rodentia: Castoridae) and cross-species amplification in the European beaver, Castor fiber // Molecular Ecology Resources. Vol.9. P.551–554.
  • Pritchard J.K., Stephens M. & Donnelly P. 2000. Inference of population structure using multilocus genotype data // Genetics. Vol.155. P.945–959.
  • Ritland K. 1996. Estimators for pairwise relatedness and individuals inbreeding coefficient // Genetic Research. Vol.67. P.175–185.
  • Ritland K. 2005. Multilocus estimation of pairwise relatedness with dominant markers // Molecular Ecology. Vol.14. P.3157–3165.
  • Senn H., Ogden R., Frosch Ch., Syruckova A., Campbell-Palmer R., Munclinger P., Durka W., Kraus R.H.S., Saveljev A.P., Nowak C., Stubbe A., Stubbe M., Michaux J., Lavrov V., Samiya R., Uleviиius A. & Rosell F. 2014. Nuclear and mitochondrial genetic structure in the Eurasian beaver Castor fiber – implications for future reintroductions // Evolutionary Applications. Vol.7. No.6. P.645–662.
  • Stocker G. 1985. Biber Castor fiber L. in der Schweiz. Probleme der Wiedereinbьrgerung aus biologischer und цkologischer Sicht. Birmensdorf: Swiss Federal Institute of Forestry Research. Bericht No.274. 149 S.
  • Suenga E. & Hakamura H. 2005. Evaluation of three methods for effective extraction of DNA from human hair // Journal of Chromatography. No.820. P.137–141.
  • Tamura K., Peterson D., Peterson N., Stecher G., Nei M. & Kumar S. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods // Molecular Biology and Evolution. Vol.28. P.2731–2739.
  • Thompson J.D., Higgins D. & Gibson T. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice // Nucleic Acids Research. Vol.22. P.4673–4680.
  • Vila C., Amorim I.R., Leonard J.A., Posada D., Castroviejo J, Petrucci-Fonseca F., Crandall K.A., Ellegren H. & Wayne K. 1999. Mitochondrial DNA phylogeography and population history of the grey wolf Canis lupus // Molecular Ecology. Vol.8. P.2089–2103.
  • Walsh P., Metzger A.D. & Higuchiet R. 1991. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material // BioTechniques. Vol.10. No.4. P.506–513.

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