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New microsatellite markers for Myotis daubentonii and Eptesicus nilssonii (Vespertilionidae, Chiroptera)
Smirnov D.G., Titov S.V., Baishev F.Z.
P. 81-83
New microsatellite markers were developed and characterized for two vespertilionid species: three loci for Myotis daubentonii (MmCCA, MdTTA, MdTTTA) and two loci for Eptesicus nilssonii (EnTCA, EnTCTA). The usefulness of these markers were assessed by screening a sample of wing membrane biopsy of 136 specimens of M. daubentonii and 206 specimens of E. nilssonii, collected in Samara Province, Penza Province and Khanty-Mansi Autonomous Area (Russia). All the loci were highly polymorphic. Both moderately and highly polymorphic loci were identified with 3-15 alleles segregating per locus (mean in M. daubentonii 3.45±0.29, in E. nilssonii 6.07±0.38). The minimal heterozygosity was found in locus MmCCA (HO=0.284±0.034, HE=0.305±0.035), while all the rest of loci had moderate and high heterozygosity. These polymorphic markers will provide a valuable tool for assessment of population genetics of these two species.


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