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Karyotype, genetic and morphological variability in North China zokor, Myospalax psilurus (Rodentia, Spalacidae, Myospalacinae)
Puzachenko A. Yu., Pavlenko M. V., Korablev V. P., Tsvirka M. V.
P. 27-46
Both genetic and morphometric methods have been applied for studying of differentiation in North China zokor, Myospalax psilurus, which includes zokors from Russian Far East (Primorsky Krai), North China, Eastern Mongolia and Russian Transbaikalia (Zabaykalsky Krai). There are two subspecies of North China zokor which were described earlier: M. p. psilurus and M. p. epsilanus. The taxonomic statuses of these forms are debated. The diploid number of chromosomes in tested zokors was 64, but there were notable differences in the karyotype structures from Zabaykalsky Krai (‘epsilanus’: 9-12 M-SM, 10-13 ST, 8-9 A:) and from Primorsky Krai (‘psilurus’: 9 M-SM, 13-14 ST, 8-9 A). These populations are clearly differed by spectra of blood serum proteins (transferrins): all samples from Primorsky Krai were found to be monomorphic by TF-B, while the samples from Zabaykalsky Krai possessed TF-C only. Marginal populations from Zabaykalsky Krai and Primorsky Krai have significant differences according to RAPD-PCR results of the study. That allows estimating these differences as corresponding to interspecies level. Genetic distance between them is high and it may be compared with the distance between another zokor species, M. aspalax and M. armandii (Pavlenko & Korablev, 2003a; Puzachenko et al., 2009; Tsvirka et al., 2011). A similar pattern of differentiation is detected by sequencing of mitochondrial markers: hypervariable region D-loop and cytochrome b (Tsvirka et al, 2009a).
The main morphological differences within the North China zokor were found out in hard palate features (foramina incisive size and construction, length of maxillary bone). According to the available data, zokors from Primorsky Krai are identical to the zokors from the southern part of the species range and are considered as typical M. p. psilurus. The zokors from the Great Khingan region must be considered as M. p. epsilanus. The zokors from Zabaykalsky Krai and Eastern Mongolia are definitely close to M. p. epsilanus by the morphometric data, but have some specific features in foramina incisiva construction, the length of the M1 and M2 and the total length of the upper tooth-row. So, we propose to consider this form as a separate subspecies of the North China zokor provisionally. For the further revision, integrated genetic and morphological research of the zokors from the Chinese part of the species range is necessary to be done.

DOI: 10.15298/rusjtheriol.13.1.04

References

  • Abramov A.V., Puzachenko A.Yu. 2005. Sexual dimorphism of craniological characters in Eurasian badgers, Meles spp. (Carnivora, Mustelidae) // Zoologischer Anzeiger. Vol.244. P.11-29.

  • Abramov A.V., Puzachenko A.Yu., Wiig Ш. 2009. Cranial variation in the European badger Meles meles (Carnivora, Mustelidae) in Scandinavia // Zoological Journal of Linnean Society. Vol.157. P.433-450.

  • Allen J.A. 1909. Mammals from Shen-Si province China // Bulletin of the American Museum of Natural History. Vol.26. P.425-430.

  • Allen G.M. 1938. The mammals of China and Mongolia // Bulletin of the American Museum of Natural History. Vol.1. Part 1. P.1-620.

  • Baryshnikov G.F., Puzachenko A.Yu. 2011. Craniometrical variability of cave bears (Carnivora, Ursidae) // Quaternary International. Vol.245. P.350-368.

  • Cai Z., Zhang T., Ci H., Tang L., Lian X., Liu J., Su J. 2007. Mitochondrial phylogeography and genetic diversity of plateau zokor (Myospalax baileyi) // Acta Zoologica Sinica. Vol.27. No.2. P.130-137.

  • Carleton M.D., Musser G.G. 2005. Order Rodentia // Wilson D.E. & Reeder D.M. (eds.). Mammal Species of the World. Third edition. Baltimore: The Johns Hopkins University Press. P.745-752.

  • Chelomina G.N., Korablev V.P., Pavlenko M.V. 2011. Genetic Diversity and phylogenetic relationschips of the Manchurian zokor Myospalax psilurus (Rodentia, Muridae) according to RAPD-PCR analysis // Biology Bulletin. No.38. P.222-230.

  • Chelomina G.N., Spiridonova L.N., Korablev V.P., Pavlenko M.V., Zhuravlev Yu.N. 2003. Rare species of the Russian Far East: RAPD-PCR analysis of genetic diversity of Amur tiger Panthera tigris altaica and Manchurian zokor Myospalax psilurus epsilanus populations // Proceedings of the IV European Congress of Mammalogy, Brno. P.80.

  • Davison M.L., Jones L.E. 1983. Special issue: multidimensional scaling and its applications // Applied Psychological Measurement. Vol.7. P.373-514.

  • Fan N.-C., Shi Y. 1982. A revision of the zokors of subgenus Eospalax // Acta Zoologica Sinica. No.2. P.83-199.

  • Flynn L.J. 2009. The antiquity of Rhizomys and independent acquisition of fossorial traits in subterranean muroids // Bulletin of the American Museum of Natural History. No.331. P.128-156.

  • Fomicheva I.I. 1973. [Certain aspects of investigation of the genetic polymorphism of transferrins and postalbumins in cattle] // Russian Journal of Genetics. No.9. P.59-66 [in Russian with English summary].

  • Ford C.E., Hamerton J.L. 1956. A colchicines hypotonic citrate squash sequence for mammalian chromosome // Stain Technology. No.31. P.247-251.

  • Frisman L.V., Vorontsov N.N. 1989. [Genogeographical variability of the long-tailed ground squirrel Citellus undulatus Pallas] // Kryukov A.P., Chelomina G.N. & Pavlenko M.V. (eds.). [The Present-Day Approaches to Studies of Variability]. Vladivostok: IBSS FEB RAS. P.43-46 [in Russian with English summary].

  • Galkina L.I., Nadeev I.V. 1980. [Some questions of morphology, distribution and history of the zokors (Rodentia, Myospalacinae) of Western Siberia] // [Fauna and Ecology of Vertebrates of Siberia]. Proceedings of the Biological Institute of the SB AS USSR. No.44. Novosibirsk: Nauka. P.162-176.

  • Gromov I.M., Erbaeva M.A. 1995. [The Mammals of Russia and Adjacent Territories. Lagomorphs and Rodents]. Sankt-Peterburg: Zoologicheskii Institut Rossiiskoi Akademii Nauk. 522 p. [in Russian].

  • Jansa S.A., Weksler M. 2004. Phylogeny of muroid rodents: relationships within and among major lineages as determined by IRBP gene sequences// Molecular Phylogenetics and Evolution. Vol.31. No.1. P.256-276.

  • Jansa S.A., Giarla T.C., Lim B.K. 2009. The phylogenetic position of the rodent genus Typhlomys and the geographic origin of Muroidea // Journal of Mammalogy. Vol.90. No.5. P.1083-1094.

  • Korablev V.P., Pavlenko M.V. 2004. [Current state and protection of the population of the Transbaikalian zokor Myospalax psilurus the Southern Russian Far East] // [Proceedings of the Siberian Zoological Conference]. Novosibirsk. P.142-143 [in Russian].

  • Korablev V.P., Pavlenko M.V. 2007a. [Cytogenetical characters and geographic distribution of zokors (Myospalax) in Transbaikalia] // [The Theriofauna of Russia and Neighboring Areas. Proceedings of the International Conference]. Moskva: Nauka. P.216 [in Russian].

  • Korablev V.P., Pavlenko M.V. 2007b. [Genetic characteristics and geographic distribution of zokors Myospalax (Rodentia) in South Transbaikalia] // [Environmental Cooperation Between Chita Oblast (Russian Federation) and Inner Mongolia (People Republic of China) in Transboundary Ecological Regions. Proceedings of the International Conference]. Chita. P.188-190 [in Russian].

  • Korablev V.P., Pavlenko M.V., Puzachenko A.Y., Tsvirka M.V. 2009. [Integrated study of zokors (Myospalax) from Russian part of the Amur Basin] // [III Readings from Druzhinin: Integrated Studies for Environment in the Amur Basin: Proceedings of the Inter-regional Researche Conference]. Khabarovsk: FEB RAS. P.198-200 [in Russian].

  • Kostenko V.A. 1970. [Manchurian zokor in Primorsky Krai] // [Small Mammals of Priamurye and Primorye]. Vladivostok: FEB RAS. P.170-175 [in Russian].

  • Kostenko V.A. 1989. [Manchurian zokor (Myospalax psilurus epsilanus Thomas, 1907)] // [Rare Vertebrate Animals of the Soviet Far East and Their Protection]. Leningrad: Nauka. P.184-185 [in Russian].

  • Kostenko V.A., Korablev V.P. 2005. [The Transbaikalian zokor - Myospalax psilurus epsilanus Thomas, 1912] // Kostenko V.A. (ed.). Krasnaya Kniga Primorskogo Kraya: Zhivotnye [Red Data Book of Primorsky Krai: Animals]. Vladivostok: Apel'sin. P.363-365 [in Russian].

  • Kruskal J.B. 1964. Multidimensional scaling by optimizing goodness of fit to a nonmetric hypothesis // Psychometrika. Vol.29. P.1-27.

  • Kupriyanova I.F., Puzachenko A.Y., Agadzanyan A.K. 2003. [Temporal and spatial patterns of craniological variation in common shrew, Sorex araneus (Insectivora)] // Zoologicheskii Zhurnal. Vol.82. No.7. P.839-851 [in Russian with English summary].

  • Lawrence M.A. 1991. A fossil Myospalax cranium (Rodentia, Muridae) from Shanxi, China, with observations on zokor relationships // Griffiths T.A. & Klingener D. (eds.). Contributions to Mammalogy in Honor of Karl F. Koopman // Bulletin of the American Museum of Natural History. No.206. P.261-286.

  • Lee M.N., Elder F.B. 1980. Yeast stimulation of bone marrow mitosis for cytogenetics investigation // Cytogenetics and Cell Genetics. No.26. P.36-40.

  • Li B.G., Chen F.G. 1987. A comparative study of the karyotypes and LDH isoenzymes from some zokors of the subgenus Eospalax, genus Myospalax // Acta Theriologica Sinica. No.4. P.275 -286.

  • Lin G.-H., Wang K., Deng X.-G., Nevo E., Zhao F., Su J.-P., Guo S.-C., Zhang T.-Z., Zhao H. 2014. Transcriptome sequencing and phylogenomic resolution within Spalacidae (Rodentia) // BMC Genomics. Vol.15. P.32.

  • Martynova L.Ya. 1975. [Population cytogenetics of zokors (Myospalacinae, Rodentia)] // Systematics and Cytogenetics of Mammals]. Moskva: Nauka. P.13-15 [in Russian].

  • Martynova L.Ya. 1976. [Chromosomal differentiation of three species of zokors (Rodentia, Myospalacidae)] // Zoologicheskii Zhurnal. Vol.55. P. 1265-1268 [in Russian with English summary].

  • Martynova L.Ya. 1978. [Comparative Cytogenetics and Species Limits of Two Groups of Fossorial Mammals]. PhD Thesis. Moscow (in Russian).

  • Martynova L.Ya., Fomicheva I.I., Vorontsov N.N. 1977. [An electrophoretic study of blood proteins in three species of zokors (Myospalacinae, Rodentia)] // Zoologicheskii Zhurnal. Vol.56. P.1538-1542 [in Russian with English summary].

  • McKenna M.C., Bell S.K. 1997. Classification of Mammals Above the Species Level. New York: Columbia University Press. 640 p.

  • Meulen A.J. van der. 1973. Middle Pleistocene smaller mammals from the Monte Peglia (Orvieto, Italy) with special reference to the phylogeny of Microtus (Arvicolidae, Rodentia) // Quaternaria. Vol.17. P.1-144.

  • Mezhzherin S.V. 1996. Genetic relationships and systematical identity of Pamiro-Alay wood mouse Apodemus sylvaticus pallipes (Rodentia, Muridae) // Biology Bulletin. Vol.23. P.30-39.

  • Mezhzherin S.V., Brandler O.V., Lyapunova E.A., Morozov-Leonov S.Y., Vorontsov N.N. 1999. [Genetic relationships and differentiation in ground squirrels Marmotinae Pocock, 1923 (Rodentia, Sciuridae) from Palearctics] // Russian Journal of Genetics. No.35. P.756-764 [in Russian with English summary].

  • Miller M.P. 1997. Tools for population genetic analysis (TFPGA) 1.3: a Windows program for the analysis of allozyme and molecular population genetic data. Computer software distributed by author.

  • Nevo E., Ivanitskaya E., Filippucci M.G., Beiles A. 2000. Speciation and adaptive radiation of subterranian mole rats, Spalax ehrenbergi superspecies, in Jordan // Biological Journal of the Linnean Society. Vol.69. P.263-281.

  • Norris R.W., Zhou K.Y., Zhou C.Q., Yang G., Kilpatrick C.W., Honeycutt R.L. 2004. The phylogenetic position of the zokors (Myospalacinae) and comments on the families of muroids (Rodentia) // Molecular Phylogenetics and Evolution. Vol.31. P.972-978.

  • Nowak R.M. 1999. Walker's Mammals of the World. Sixth edition. Baltimore: the Johns Hopkins University Press. 936 p.

  • Ognev S.I. 1947. [Animals of the USSR and Adjacent Countries]. Vol.5. [Rodents]. Moskva: Akademiya Nauk SSSR. 809 p. [in Russian].

  • Pasteur N., Pasteur G., Bonhomme F., Catalan J., Britton-Davidian J. 1988. Practical Isozyme Genetics. Chichester: Halsted Press. 215 p.

  • Pavlenko M.V., Korablev V.P. 1999. [Genetic typing of Manchurian zokor Myospalax psilurus] // [Proceedings of the 6th Congress of Theriological Society of Russia]. Moskva: Severtsov Institute of Ecology and Evolution. P.188 [in Russian].

  • Pavlenko M.V., Korablev V.P. 2003a. [Genetic differentiation, systematic position and problem of conservation of the Manchurian zokor Myospalax psilurus (Rodentia, Myospalacinae) marginal population] // [The Theriofauna of Russia and Neighboring Areas. Proceedings of the International Conference]. Moscow: Severtsov Institute of Ecology and Evolution. P.51 [in Russian].

  • Pavlenko M.V., Korablev V.P. 2003b. [Genetic differentiation, systematic position and conservation of peripheral populations of Manchurian zokor Myospalax psilurus (Rodentia, Myospalacinae)] // Kryukov A.P. & Yakimenko L.V. (eds.). [Problems of Evolution]. Vol.5. Vladivostok: Dal'nauka. P.167-177 [in Russian with English summary].

  • Pavlenko M.V., Korablev V.P. 2005. [Genetic diversity and geographic distribution of zokor Myospalax (Rodentia) in southern Transbaikalia] // [Ecosystems of Mongolia and Frontier Areas of Adjacent Countries: Natural Resources, Biodiversity and Ecological Prospects. Proceedings of the International conference]. Ulaanbaatar: Publishing House 'Bembi San'. P.300-302 [in Russian].

  • Pavlenko M., Korablev V., Tsvirka M., Pang J. 2008. Genetic differentiation of zokors (Rodentia, Myospalacinae) from East Russia: protein electrophoretic markers, karyological and molecular data // Eleventh International Conference on Rodent Biology, Rodens et Spatium. Myshkin. P.57.

  • Pavlinov I.Ya., Rossolimo O.L. 1987. [Systematics of Mammals of the USSR]. Moskva: MGU. 258 p. [in Russian].

  • Pavlinov I.Ya., Rossolimo O.L. 1998. [Systematics of Mammals of the USSR: Addition]. Moskva: MGU. 190 p. [in Russian].

  • Puzachenko A.Y. 2001. [Skull variability in the common mole rat Spalax microphthalmus (Spalacidae, Rodentia). 1. A method for analysis of data, non-age variability in males] // Zoologicheskii Zhurnal. Vol.80. No.3. P.343-357 [in Russian with English summary].

  • Puzachenko A.Y. 2006. [Variability of the skull in the lesser mole rats, Nannospalax Palmer, 1903 (Rodentia)] // Zoologicheskii Zhurnal. Vol.85. No.2. P.235-253 [in Russian with English summary].

  • Puzachenko A.Y., Pavlenko M.V., Korablev V.P. 2009. [Variability of skulls in zokors (Rodentia, Myospalacidae)] // Zoologicheskii Zhurnal. Vol.88. No.1. P.92-112 [in Russian with English summary].

  • Puzachenko A.Y., Pavlenko M.V., Korablev V.P., Tsvirka M.V. 2011. [Armand's zokor Myospalax armandii (Milne-Edwards, 1867) - new species in fauna of Russia] // [The Theriofauna of Russia and Neighboring Areas. Proceedings of the International Conference]. Moskva: Nauka. P.387 [in Russian].

  • Saitou N., Nei M. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees // Molecular Biology and Evolution. Vol.4. P.406-425.

  • Sambrook J., Fritsch E.F., Maniatis T. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.

  • Searle S.R., Casella G. & McCulloch C.E. 1992. Variance Components. New York: John Wiley & Sons. 501 p.

  • Smith A.T., Xie Y. 2008. A Guide to the Mammals of China. Princeton and Oxford: Princeton University Press. 544 p.

  • Sneath P.H.A., Sokal R.R. 1973. Numerical Taxonomy: the Principles and Practice of Numerical Classification. San Francisco: Freeman. 573 p.

  • Tsvirka M., Pavlenko M., Korablev V., Pang J. 2009a. Molecular-genetic relationships of the zokors (Rodentia, Myospalacinae): analysis of D-loop region polymorphism // Modern Achievements in Population, Evolutionary and Ecological Genetics (MAPEEG - 2009). Proceedings of the International Symposium. Vladivostok. P.48.

  • Tsvirka M.V., Pavlenko M.V., Korablev V.P. 2009b. [Genetic differentiation and phylogenetics relations of zokors (Rodentia, Myospalacinae) inferred from molecular-genetic analysis] // [Proceedings of 5th Meeting of Vavilov Society of Genetics and Breeders]. Part 2. Moskva: Rossiiskii Gosudarstvennyi Agrarnyi Universitet. P.307 [in Russian].

  • Tsvirka M.V., Pavlenko M.V., Korablev V.P. 2011. Genetic diversity and phylogenetic relationships in the zokor subfamily Myospalacinae (Rodentia, Muridae) inferred from RAPD-PCR // Russian Journal of Genetics. Vol.47. No.2. P.205-215.

  • Van de Peer Y., De Wacher R. 1994. TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment // Computer Applications in the Biosciences. No.10. P.569-570.

  • Vorontsov N.N., Martynova L.Ya. 1976. [Population cytogenetics of Myospalax myospalax Laxm. (Rodentia, Myospalacidae)] // Doklady Akademii Nauk. Vol.230. P.447-449 [in Russian].

  • Vorontsov N.N., Frisman L.V., Nadler C.F., Lyapunova E.A., Hoffman R.S., Fomicheva I.I. 1978. [Population genetics and genogeography of wild animals. I. Genogeography of transferrins and variants of glucose-6-phosphate-dehydrogenase in populations of Palaearctic long-tailed ground squirrels Citellus (=Spermophilis) undulates] // Russian Journal of Genetics. Vol.14. P.805-816 [in Russian with English summary].

  • Yeh F.C., Boyle T.J.B. 1997. Population genetic analysis of co-dominant and dominant markers and quantitative traits // Belgian Journal of Botany. Vol.129. P.159.

  • Zheng S., Cai B. 1991. Fossil micromammals from the Donggou section of Dongyaozitou, Yuxian County, Hebei Province // Contribution to INQUA XIII. P.100-131.

  • Zhang Y.S., Jin G., Quan S., Li Z., Ye F., Wang Zhang M. 1997. Distribution of Mammalian Species in China. Beijing: China Forestry Publishing House. 394 p.

  • Zheng Sh. 1994. Classification and evolution of the Siphneidae // Tomida Y.C. & Setoguchi Li T. (eds.). Rodent and Lagomorph Families of Asian Origins and Diversification. National Science Museum Monographs, No.8. Tokyo: National Science Museum. P.57-76.

  • Zhou C., Zhou K. 2008. The validity of different zokor species and the genus Eospalax inferred from mitochondrial gene sequences // Integrative Zoology. Vol.3. No.4. P.290-298.

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