Russian Journal of Theriology. Main page    

Russian Journal of Theriology. Главная страница
Доступ к статьям
Электронная подача статей, правила для авторов и тп
Вход для рецензентов
Здесь можно подписаться на новостную рассылку RJT
Контактная информация

English version

Bats prey on amphipods of Lake Baikal: morphological and genetic evidence
Botvinkin A.D., Mekhanikova I.V., Adelshin R.V., Peretolchina T.E., Romanova E.V.
P. 81-91
Study aims to assess the prevalence of endemic amphipods (Crustacea, Gammaroidea, Amphipoda) in the diet of insectivorous bats (Chiroptera) feeding over the surface of the unique freshwater Lake Baikal. In 2020–2023, 41 samples of bat faeces (mainly Myotis petax) were collected at 14 sampling sites in the water area, on the shore and islands of Lake Baikal. The body fragments of amphipods in faecal samples were identified using a light microscope. DNA was extracted from collected faecal samples and screened for presence of pelagic amphipod species Macrohectopus branickii. A fragment of the COI mitochondrial gene was amplified in three rounds of nested PCR. All newly sequenced fragments demonstrated 92–100% identity to sequences of the same species deposited in GenBank. DNA of Ma. branickii was detected in five of 41 samples (12.2%). Microscopic analysis revealed body fragments of Ma. branickii and of three benthic amphipod species (Micruropus wohlii wohlii, Mi. wohlii platycercus, and Gmelinoides fasciatus) as well as unidentified amphipod fragments in six of 18 samples (33.3%) Amphipod remains were detected in small amounts in many examined samples, but insect remains visually predominated at microscopy. Thus, the use of molecular and morphological approaches facilitated identification of remains of four amphipod species in samples from seven of 14 sampling sites. Direct evidence was received for new trophic interactions in Lake Baikal ecosystem: bat My. petax feeds on several species of endemic amphipods catching them during periodic nocturnal ascents to the water surface.

DOI: 10.15298/rusjtheriol.24.2.01

Литература

  • Aizpurua O., Garin I., Alberdi A., Salsamendi E., Baagøe H. & Aihartza J. 2013. Fishing long-fingered bats (Myotis capaccinii) prey regularly upon exotic fish // PLoS One. Vol.8. No.11. P.e80163. DOI: 10.1371/journal.pone.0080163

  • Ahlén I., Baagøe H.J. & Bach L. 2009. Behavior of Scandinavian bats during migration and foraging at sea // Journal of Mammalogy. Vol.90. No.6. P.1318–1323. DOI: 10.1644/09-MAMM-S-223R.1

  • Batranin D.A., Takhteev V.V., Eropova I.O. & Govorukhina E.B. 2019. [Structure of the nocturnal migratory complex of hydrobionts in different parts of Lake Baikal in the summer of 2017 and the problem of the changes in the trophic status of the Lake] // Izvestiya Irkutskogo gosudarstvennogo universiteta. Seriya biologiya, ekologiya. Vol.27. P.62–86. DOI: 10.26516/2073-3372.2019.27.62 [in Russian with English summary].

  • Bazikalova A.Ya. 1962. [Taxonomy, ecology, and distribution of the genera Micruropus Stebbing and Pseudomicruropus nov. gen. (Amphipoda, Gammaridea)] // [Systematics and Ecology of the Crustaceans of Lake Baikal]. Trudy Limnologicheskogo Instituta. Vol.2(22). Part 1. P.3–140 [in Russian].

  • Bekman M.Yu.& Afanasyeva E.L. 1977. [Distribution and production of Macrohectopus in Lake Baikal] // Bekman M.Yu. (ed.). Trudy Limnologicheskogo Instituta. Vol.19. Biologicheskaya Produktivnost’ Pelagiali Bajkala i ee Izmenchivost’. Novosibirsk: Nauka. P.76–98 [in Russian].

  • Boonman A.M., Boonman A.M., Bretschneider F. & van de Grind W.A. 1998. Prey detection in trawling insectivorous bats: duckweed affects hunting behaviour in Daubenton’s bat, Myotis daubentonii // Behavioral Ecology and Sociobiology. Vol.44. P.99–107. DOI: 10.1007/s002650050521.

  • Borisenko A.V. 1999. A mobile trap for capturing bats in flight // Plecotus et al. Vol.2. P.3–19 [in Russian with English summary].

  • Botvinkin A.D., Adelshin R.V.& Peretolchina T.E. 2023. [Bats (Chiroptera) over Lake Baikal: experience of observations from board the ship] // Baykal’skiy Zoologicheskii Zhurnal. Vol.3. No.35. P.65–72 [in Russian with English summary].

  • Botvinkin A.D., Klopova A.A., Mekhanikova I.V., Romanova E.V., Shilenkov V.G., Rudakov D.M. & Samusyonok V.P. 2024. First data about the dietary pattern of the eastern bat Myotis petax (Hollister, 1812) feeding near Lake Baikal based on visual analysis of feces (using coproscopy data) // Contemporary Problems of Ecology. Vol.4. P.566–573. DOI: 10.1134/S1995425524700367

  • Clare E.L., Symondson W.O., Broders H., Fabianek F., Fraser E.E., MacKenzie A., Boughen A., Hamilton R., Willis C.K., Martinez-Nuñez F., Menzies A.K., Norquay K.J., Brigham M., Poissant J., Rintoul J., Barclay R.M. & Reimer J.P. 2014. The diet of Myotis lucifugus across Canada: assessing foraging quality and diet variability // Molecular Ecology. Vol.23. No.15. P.3618–3632. DOI: 10.1111/mec.12542.-6

  • Compson Z.G., McClenaghan B., Singer G.A.C., Fahner N.A. & Hajibabaei M. 2020. Metabarcoding from microbes to mammals: comprehensive bioassessment on a global scale // Frontiers in Ecology and Evolution. Vol.8. P.e581835. DOI: 10.3389/fevo.2020.581835

  • Corduneanu A., Wu-Chuang A., Maitre A., Obregon D., Sándor A.D. & Cabezas-Cruz A. 2023. Structural differences in the gut microbiome of bats using terrestrial vs. aquatic feeding resources // BMC Microbiology. Vol.3. No.1. P.93. DOI: 10.1186/s12866-023-02836-7

  • Didorenko S.I., Botvinkin A.D. & Takhteev V.V. 2021. A new, original trophic relationship in the Lake Baikal ecosystem: the pelagic amphipod, Macrohectopus branickii (Crustacea, Amphipoda) and Myotis petax bats (Mammalia, Chiroptera) // Biology Bulletin. Vol.48. No.7. P.907–914. DOI: 10.1134/S1062359021070116

  • Didorenko S.I., Botvinkin A.D. & Takhteev V.V. 2022. Myotis petax (Chiroptera, Vespertilionidae) preys on pelagic Amphipoda (Crustacea, Gammaroidea) of Lake Baikal // Acta Chiropterologica. Vol.24. No.1. P.187–194. DOI: 10.3161/15081109ACC2022.24.1.015

  • Drinkwater R., Goodwin A., Cush J., Korstian J.M., Chumchal M.M., Herrera L.G., Valdez M., Otálora-Ardila A., Flores Martinez J.J. & Clare E.L. 2021. Molecular diet analysis of the marine fish-eating bat (Myotis vivesi) and potential mercury exposure // Canadian Journal of Zoology. Vol.99. No.9. P.752–759. DOI: 10.1139/cjz-2021-0018

  • Dybowsky B.N. 1874. Beiträge zur näheren Kenntnis der in dem Baikal-See vorkommenden niederen Krebse aus der Gruppe der Gammariden [Contributions to the closer knowledge of the lower crustaceans from the group of gammarids found in Lake Baikal]. Herausgegeben von der Russischen Entomologischen Gesellschaft zu St. Petersburg. Buchdrucke Von W. Besobrasoff & Comp. 190 p.

  • Hornok S., Estók P., Kováts D., Flaisz B., Takács N., Szőke K., Krawczyk A., Kontschán J., Gyuranecz M., Fedák A., Farkas R., Haarsma A.J. & Sprong H. 2015. Screening of bat feces for arthropod-borne apicomplexan protozoa: Babesia canis and Besnoitia besnoiti-like sequences from Chiroptera // Parasites & Vectors. Vol.8. P.e441. DOI: 10.1186/s13071-015-1052

  • Jiang T.L., Feng J., Csorba G. & Bates P. 2019. Myotis pilosus // IUCN Red List of Threatened Species: e.T14193A22062554. DOI: 10.2305/IUCN.UK.2019-3 RLTS.T14193A22062554.en

  • Karnaukhov D.Yu., Bedulina D.S., Kaus A., Prokosov S.O., Sartoris L., Timofeyev M.A. & Takhteev V.V. 2016. Behaviour of Lake Baikal amphipods as a part of the night migratory complex in the Kluevka settlement region (South-Eastern Baikal) // Crustaceana. Vol.89. No.4. P.419–430. DOI: 10.1163/15685403-00003530

  • Kalko E.K.V. & Schnitzler H.U. 1989. The echolocation and hunting behavior of Daubenton’s bat, Myotis daubentoni // Behavioral Ecology and Sociobiology. Vol.24. P.225–238. DOI: 10.1007/BF00295202

  • Letko M., Seifert S.N., Olival K.J., Plowright R.K. & Munster V.J. 2020. Bat-borne virus diversity, spillover and emergence. Nature reviews // Microbiology. Vol.18. No.8. P.461–471. DOI: 10.1038/s41579-020-0394-z

  • Mekhanikova I.V. 2021. Calceoli: antennal sensory organs of amphipods (Crustacea, Amphipoda, Gammaridea) from Lake Baikal // Biology Bulletin. Vol.48. No.8. P.1250–1262. DOI:10.1134/S1062359021080215

  • Mekhanikova I.V. & Takhteev V.V. 2001. [Diel vertical migrations of amphipods of Lake Baikal: possible causes and ecological significance] // Sbornik nauchnyh trudov. Issledovanie fauny vodoemov Vostochnoj Sibiri. Irkutskij Gosudarstvennyj Universitet. P.88–108 [in Russian with English summary].

  • Otalora-Ardila A., Herrera M.L.G., Flores-Martinez J.J. & Voigt C.C. 2013. Marine and terrestrial food sources in the diet of the fish-eating myotis (Myotis vivesi) // Journal of Mammalogy. Vol.94. No.5. P.1102–1110. DOI: 10.1644/12-MAMM-A-281.1

  • Owczarzy R., Tataurov A.V., Wu Y., Manthey J. A., McQuisten K.A., Almabrazi H.G., Pedersen K.F., LinY., Garretson J., McEntaggart N.O., Sailor C.A., Dawson R.B. & Peek A.S. 2008. IDT Sci Tools: a suite for analysis and design of nucleic acid oligomers // Nucleic Acids Research. Vol.36. (Web Server issue) P.W163–W169. DOI: 10.1093/nar/gkn198

  • Robson S.R. 1984. Myotis adversus (Chiroptera: Vespertilionidae): Australia’s fish-eating bat // Australian Mammalogy. Vol.7. P.51–52.

  • Romanova E.V., Bukin Y.S., Mikhailov K.V., Logacheva M.D., Aleoshin V.V. & Sherbakov D.Y. 2021. The mitochondrial genome of a freshwater pelagic amphipod Macrohectopus branickii is among the longest in Metazoa // Genes. Vol.12. No.12. P.2030. DOI: 10.3390/genes12122030

  • Rusinek O.T., Takhteev V.V., Khodzer T.V. et al. 2012. Baikalovedenie [Baikal Studies]. Novosibirsk: Nauka. Vol.2(2). 644 p. [in Russian].

  • Siemers B.M., Dietz C., Nill D. & Schnitzler H.-U. 2001a. Myotis daubentonii is able to catch small fish // Acta Chiropterologica. Vol.3. No.1. P.71–75.

  • Siemers B.M., Stilz P. & Schnitzler H.U. 2001b. The acoustic advantage of hunting at low heights above water: behavioural experiments on the European “trawling” bats Myotis capaccinii, M. dasycneme and M. daubentonii // Journal of Experimental Biology. Vol.204. No.22. P.3843–3854. DOI: 10.1242/jeb.204.22.3843

  • Shiveley R., Barboza P., Doak P. & Jung T.S. 2018. Increased diet breadth of little brown bats (Myotis lucifugus) at their northern range limit: a multimethod approach // Canadian Journal of Zoology. Vol.96. No.1. P.31–38. DOI: 10.1139/cjz-2017-0017

  • Sommer R.S., Hofreiter M., Kruger F., Siemers B.M., Paijmans J.L.A., Li Chenhong & Geiger M.F. 2019. Preliminary results on the molecular study of fish-eating by ‘trawling Myotis’ bat species in Europe // Vertebrate Zoology. Vol.69. No.1. P.83–92. DOI: 10.26049/VZ69-1-2019-03

  • Sowinsky V.K. 1915. [Amphipoda of Lake Baikal] // Zoologicheskiye issledovaniya Baykala. Vol.9. No.1. P.1–381 [in Russian].

  • Stenhouse E.H., Bellamy P., Kirby W., Vaughan I.P., Drake L.E., Marchbank A., Workman T., Symondson W.O.C. & Orozco-ter Wengel P. 2023. Multi-marker DNA metabarcoding reveals spatial and sexual variation in the diet of a scarce woodland bird // Ecology and Evolution. Vol.13. No.5. P.e10089. DOI: 10.1002/ece3.10089

  • Takhteev V.V. 2000. [Essays on the amphipods of Lake Baikal (systematics, comparative ecology, evolution)]. Irkutsk: Izdatel’stvo Irkutskogo Universiteta. 355 p. [in Russian with English summary]

  • Takhteev V.V, Berezina N.A. & Sidorov D.A. 2015. Checklist of the Amphipoda (Crustacea) from continental waters of Russia, with data on alien species // Arthropoda Selecta. Vol.24. No.3. P.335–370.

  • Takhteev V.V., Karnaukhov D.Yu., Govorukhina E.B. & Misharin AS. 2019. Diel vertical migrations of hydrobionts in the coastal area of Lake Baikal // Inland Water Biology. Vol.12. No.2. P.178–189. DOI: 10.15298/arthsel.24.3.09

  • Thong V.D., Denzinger A., Long V., Sang N.V., Huyen N.T.T., Thien N.H., Luong N.K., Luong N.K., Tuan L.Q., Ha N.M., Luong N.T. & Schnitzler H.U. 2022. Importance of mangroves for bat research and conservation: A case study from Vietnam with notes on echolocation of Myotis hasselti // Diversity. Vol.14. No.4. P.258. DOI: 10.3390/d14040258

  • Timoshkin O.A., Mekhanikova I.V. & Shubenkov S.G. 1995. [Pelagic amphipods — M. branickii (Dyb.)] // Mazepova G.F., Timoshkin O.A., Melnik N.G., Oblokina L.A. & Tanichev A.I. (eds.). [Guide and Key to Pelagobionts of Baikal with Short Ecological Notes]. Novosibirsk: Nauka. P.480–522 [in Russian].

  • Vesterinen E.J., Lilley T., Laine V.N. & Wahlberg N. 2013. Next generation sequencing of fecal DNA reveals the dietary diversity of the widespread insectivorous predator Daubenton’s bat (Myotis daubentonii) in Southwestern Finland // PLoS ONE. Vol.8. No.11. P.e82168. DOI: 10.1371/journal.pone.0082168

  • Zaidykov I.Y., Naumova E.Y. & Sukhanova L.V. 2023. MtDNA polymorphism of Macrohectopus branickii Dybowsky, 1974 (Amphipoda) — an endemic pelagic key species of Lake Baikal // Chaplina T. (ed.). Complex Investigation of the World Ocean (CIWO-2023). Springer Proceedings in Earth and Environmental Sciences. P.223–229.

Скачать PDF